Cholesterol maintains sperm plasma membrane stability during the cooling process.. The addition of this essential lipid component makes BotuSemen Special ideal for pre-freezing centrifugation. BotuSemen Special is suitable for all stallion, especially for those with spermatozoa that are sensitive to cooled transport.
How to Prepare:
Carefully open the sachet, taking care not to touch the inside of the packet with your fingers, and empty the contents of the sachet into the bottle of sterile water. Replace lid on bottle, and shake well. Wait 15 minutes prior to using. This allows the cholesterol to distribute properly for optimal results. Warm to 37°C before extending semen. Be sure extender remains at the same temperature as the semen to prevent cold shock of the semen.
Usage:
Extend 1:1 (V/V) wait 15 minutes for insemination with no cooled semen.
Use dilutions of 30 to 50 million/ml for cooled transport. Extend 1:1 (v/v and wait 15 minutes for an insemination with non-cooled semen. In case of samples requiring centrifugation before cooled transport, resuspend the sperm at a rate of between 30 to 50 million/ml
Contains Penicillin K 1 g/l and Gentamicine 1 g/l.
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